Projects
NUEVA METODOLOGÍA ANALÍTICA PARA LA CARACTERIZACIÓN DE BIOACEITES Y COMBUSTIBLES
Identificación y cuantificación de biomarcadores en muestras de sangre de pacientes con enfermedades de depósito lisosomal (enfermedades raras) mediante técnicas analíticas avanzadas. (LISA)
Las enfermedades de depósito lisosomal (EDL) son un grupo de enfermedades raras que suponen un grave problema clínico, económico y social. Las técnicas actuales de medición de la actividad enzimática residual de las EDL no permiten diagnosticar y evaluar la progresión de las mismas. Por otro lado, las técnicas cromatográficas convencionales implican largos tiempos de análisis, el uso de un alto volumen de sangre y altos costes. El proyecto LISA, donde participan el CSIC (IP), La Fundación Española para el Estudio y Tratamiento de la enfermedad de Gaucher y otras lisosomales (FEETEG), la Universidad de Zaragoza y la empresa suiza CAMAG, tiene como objetivo principal la determinación de diferentes esfingolípidos considerados como biomarcadores de enfermedades de depósito lisosomal (EDL). Este objetivo se logrará mediante el desarrollo de: (i) una plataforma basada en tarjetas de muestra de sangre seca (DBS) acoplada a Cromatografía Líquida y Espectrometría de Masas (LC-MS) para llevar a cabo una rápida, cuantitativa y simultánea determinación de los diferentes liso-esfingolipidos con gran ahorro de muestra y mejores condiciones de extracción para los pacientes. Se utilizará una amplia colección de tarjetas DBS almacenadas en la Fundación FEETEG. (ii) Una plataforma automatizada basada en Cromatografía en Capa Fina de Alta Eficacia acoplada a Espectrometría de Masas (HPTLC-MS) que sirve de apoyo a la tecnología anterior.
Desarrollo de nuevas terapias y nanobiosensores para el diagnóstico y tratamiento de colitis ulcerosa y cáncer colorectal
Articles
Berberine: A fluorescent alkaloid with a variety of applications from medicine to chemistry
ISSN: 1570-193X
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-77958167483&partnerID=65&md5=10005169c80c3e542aa0832a6b7299ad
Improved analysis of a gas oil using a high-performance thin-layer chromatographic system
ISSN: 0021-9665
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-0034310798&partnerID=65&md5=f6347066721f51d9d9e01bfebd015f2f
Sensitive and quantitative group-type analysis (Alkanes-Naphthenes-Aromatics-Polars) of a wide range of petroleum products using berberine-induced fluorescence densitometry in thin-layer chromatographic systems
ISSN: 0569-3799
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-0344118921&partnerID=65&md5=43cde05ff8d67192f724d992c18b9923
Planar chromatography for the hydrocarbon group type analysis of petroleum middle distillates and coal-derived products
ISSN: 0378-3820
DOI: 10.1016/S0378-3820(02)00011-5
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-0037141976&partnerID=65&md5=470bba1513685a5e4ccd6a11558be698
Application of Thin-Layer Chromatography with Fluorescence Scanning Densitometry for analysing saturates in heavy liquids derived from co-pyrolysis of biomass and plastics
ISSN: 0009-5893
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-0036148811&partnerID=65&md5=3563709baf7880826575445f0c8f2c2e
Hydrocarbon group-type analysis by thin layer chromatography and scanning densitometry
ISSN: 0933-4173
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-0036659851&partnerID=65&md5=84110df83459e65a04425a6b2fc79a00
Improved separation and quantitative determination of hydrocarbon types in gas oil by normal phase high-performance TLC with UV and fluorescence scanning densitometry
ISSN: 1615-9306
DOI: 10.1002/jssc.200301465
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-0346998259&partnerID=65&md5=723b80a58b97468cbcb8df9b26a116b5
General contribution of nonspecific interactions to fluorescence intensity
ISSN: 0003-2700
DOI: 10.1021/ac058045b
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-33744919300&partnerID=65&md5=79531c87e2a5241aae71c39547c9f0f2
Fluorescence resonance energy transfer in single enzyme molecules with a quantum dot as donor
ISSN: 0175-7571
DOI: 10.1007/s00249-008-0351-7
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-51849137794&partnerID=65&md5=966e66779ffe48040473f9d09a8451da
Quantum dots for single-pair fluorescence resonance energy transfer in membrane integrated EF0F1
ISSN: 0300-5127
DOI: 10.1042/BST0361017
Immobilization of the H+-ATPsynthase on glass surface and single molecule fluorescence
ISSN: 0005-2728
RESPONSES OF FOSSIL-FUEL RELATED, NONFLUORESCENT ANALYTES IN PLANAR CHROMATOGRAPHY AND HPLC-GPC USING FLUORESCENCE DETECTION BY INTENSITY CHANGES
ISSN: 0569-3799
Observation of rotation of subunit c in the membrane integrated EF0F1 by single molecule fluorescence
ISSN: 0005-2728
DOI: 10.1016/j.bbabio.2008.05.057
Caspase-dependent inhibition of mousepox replication by gzmB
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0007512
Fluorescence Detection by Intensity Changes for Automated Multiple Development-HPTLC separation of lipids
ISSN: 0021-9673
LINK: doi:10.1016/j.chroma.2010.11.033
Changes in fluorescent emission of cationic fluorophores in the presence of n-alkanes and alcohols in different polarity solvents
ISSN: 0009-2614
DOI: 10.1016/j.cplett.2010.11.004
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-78650846819&partnerID=65&md5=ee1d2b4386f34a5000134c9778b31ddf
Fast and sensitive detection of the mycotoxin gliotoxin and related compounds during human aspergillosis
ISSN: 1469-0691
DOI: 10.1111/j.1469-0691.2011.03558.x
LINK: http://onlinelibrary.wiley.com/doi/10.1111/j.1469-0691.2011.03558.x/abstract
Changes in fluorescent emission of cationic fluorophores in the presence of n-alkanes and alcohols in different polarity solvents
ISSN: 0009-2614
DOI: 10.1016/j.cplett.2010.11.004
Quantum dot bioconjugates: emerging tools with great potential to study protein interactions and dynamics by FRET
ISSN: 1756-0799
DOI: 10.1504/IJBNN.2011.038467
Fluorescence Detection by Intensity Changes for Automated Multiple Development-HPTLC separation of lipids
ISSN: 0021-9673
Fluorescence detection by intensity changes for high-performance thin-layer chromatography separation of lipids using automated multiple development
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2010.11.033
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-79954629815&partnerID=65&md5=27a40b7051a7708326e77ca588ff6e4b
Changes in Fluorescent Emission Due to Non-covalent Interactions as a General Detection Procedure for Thin-Layer Chromatography
ISSN: 1439-4235
DOI: 10.1002/cphc.201100590
Bis(methyl)gliotoxin proves to be a more stable and reliable marker for invasive aspergillosis than gliotoxin and suitable for use in diagnosis
ISSN: 0732-8893
DOI: 10.1016/j.diagmicrobio.2012.01.012
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-84860753912&partnerID=65&md5=8d6850d9f474bb2d321321dae65a55ce
Revisiting Molecular Weight Distribution of Polystyrenes Using Adsorption High-Performance Thin-Layer Chromatography
ISSN: 0933-4173
DOI: 10.1556/JPC.26.2013.1.1
LINK: http://www.akademiai.com/content/kl8884n573646100/fulltext.pdf?page=1
Recombinant production of human ICAM-1 chimeras by single step on column refolding and purification
ISSN: 1359-5113
DOI: 10.1016/j.procbio.2013.03.006
Blinking effect and the use of quantum dots in single molecule spectroscopy
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2012.10.140
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-84872406729&partnerID=65&md5=75daf33bfee9e9f2e06c1b057b2f9f66
FRET based quantification and screening technology platform for the interactions of leukocyte function-associated antigen-1 (LFA-1) with InterCellular adhesion molecule-1 (ICAM-1)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0102572
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-84904480125&partnerID=65&md5=bfffdae5ee0bd641ca30b764bfda7716
The effect of acidic pH on the inhibitory efficacy of peptides against the interaction ICAM-1/LFA-1 studied by surface plasmon resonance (SPR)
ISSN: 0956-5663
DOI: 10.1016/j.bios.2014.01.008
LINK: http://www.scopus.com/inward/record.url?eid=2-s2.0-84893169475&partnerID=65&md5=a0da10b5ef830fa703526c0f6ddfc3a5
Human NK cells activated by EBV+ lymphoblastoid cells overcome anti-apoptotic mechanisms of drug resistance in haematological cancer cells
ISSN: 2162-402X
DOI: 10.4161/2162402X.2014.991613
LINK: http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84954481360&origin=inward
Human NK cells activated by EBV+ lymphoblastoid cells overcome anti-apoptotic mechanisms of drug resistance in haematological cancer cells
ISSN: 2162-4011
Nº de artículo: e991613
Graphene quantum dots from fishbone carbon nanofibers
ISSN: 2046-2069
DOI: 10.1039/c6ra09679d
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84971222300&origin=inward
Clinical validity of bis(methylthio)gliotoxin for the diagnosis of invasive aspergillosis
ISSN: 1432-0614
DOI: 10.1007/s00253-015-7209-6
LINK: http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84958840295&origin=inward
Disseminated aspergillosis in an immunocompetent patient with detectable bis(methylthio)gliotoxin and negative galactomannan
ISSN: 1130-1406
DOI: 10.1016/j.riam.2016.05.007
Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo
ISSN: 1743-8977
DOI: 10.1186/s12989-017-0222-4
Nº de artículo: 41
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85032338650&origin=inward
Disseminated aspergillosis in an immunocompetent patient with detectable bis(methylthio)gliotoxin and negative galactomannan
ISSN: 1130-1406
LINK: https://www.scopus.com/record/display.uri?eid=2-s2.0-85008155443&origin=resultslist&sort=plf-f&src=s&st1=Disseminated+aspergillosis+in+an+immunocompetent&st2=&sid=e785b48e7b9abf81e8566d2394721549&sot=b&sdt=b&sl=63&s=TITLE-ABS-KEY%28Disseminated+aspergillosis+in+an+immunocompetent%29&relpos=2&citeCnt=0&searchTerm=
A functional analysis on the interspecies interaction between mouse LFA-1 and human intercellular adhesion molecule-1 at the cell level
ISSN: 1664-3224
DOI: 10.3389/fimmu.2017.01817
Nº de artículo: 1817
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85038943967&origin=inward
Granzyme A Contributes to Inflammatory Arthritis in Mice Through Stimulation of Osteoclastogenesis
ISSN: 2326-5205
DOI: 10.1002/art.39857
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010888096&origin=inward
Production of the invasive aspergillosis biomarker bis(methylthio)gliotoxin Within the genus aspergillus: In vitro and in vivo metabolite quantification and genomic analysis
ISSN: 1664-302X
DOI: 10.3389/fmicb.2018.01246
Nº de artículo: 1246
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85048523543&origin=inward
Preparations for invasion: Modulation of host lung immunity during pulmonary aspergillosis by gliotoxin and other fungal secondary metabolites
ISSN: 1664-3224
DOI: 10.3389/fimmu.2018.02549
Nº de artículo: 02549
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85056341107&origin=inward
Expansion of allogeneic NK cells with efficient antibody-dependent cell cytotoxicity against multiple tumors
ISSN: 1838-7640
DOI: 10.7150/thno.25149
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85049233075&origin=inward
Intracellular delivery of biologically-active fungal metabolite gliotoxin using magnetic nanoparticles
ISSN: 1996-1944
DOI: 10.3390/ma12071092
Nº de artículo: 1092
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85065676873&origin=inward
The Multifaceted Function of Granzymes in Sepsis: Some Facts and a Lot to Discover
ISSN: 1664-3224
DOI: 10.3389/fimmu.2020.01054
Nº de artículo: 1054
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85087158597&origin=inward
Intratumoral Versus Circulating Lymphoid Cells as Predictive Biomarkers in Lung Cancer Patients Treated with Immune Checkpoint Inhibitors: Is the Easiest Path the Best One?
ISSN: 2073-4409
DOI: 10.3390/cells9061525
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85087098529&origin=inward
Microbiota and Lung Cancer. Opportunities and Challenges for Improving Immunotherapy Efficacy
ISSN: 2234-943X
DOI: 10.3389/fonc.2020.568939
Nº de artículo: 568939
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85092746728&origin=inward
From tumor mutational burden to blood T cell receptor: Looking for the best predictive biomarker in lung cancer treated with immunotherapy
ISSN: 2072-6694
DOI: 10.3390/cancers12102974
Nº de artículo: 2974
LINK: https://www.scopus.com/record/display.uri?eid=2-s2.0-85092932751&origin=resultslist&sort=plf-f&src=s&st1=&st2=&sid=12c1302a8f79477b15402ca0b96804c5&sot=b&sdt=b&sl=29&s=DOI+%2810.3390%2fcancers12102974%29&relpos=0&citeCnt=0&searchTerm=
Extracellular Granzyme A Promotes Colorectal Cancer Development by Enhancing Gut Inflammation
ISSN: 2211-1247
DOI: 10.1016/j.celrep.2020.107847
Nº de artículo: 107847
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85087384727&origin=inward
Inflammatory cell death induced by cytotoxic lymphocytes: a dangerous but necessary liaison
ISSN: 1742-4658
DOI: 10.1111/febs.16093
LINK: https://www.scopus.com/record/display.uri?eid=2-s2.0-85110299225&origin=resultslist&sort=plf-f&src=s&st1=10.1111%2ffebs.16093&sid=daf2dc5cc7c9524d0f10c07570750293&sot=b&sdt=b&sl=23&s=DOI%2810.1111%2ffebs.16093%29&relpos=0&citeCnt=2&searchTerm=
Determination of the concentration of igg against the spike receptor-binding domain that predicts the viral neutralizing activity of convalescent plasma and serum against sars-cov-2
ISSN: 2079-7737
DOI: 10.3390/biology10030208
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85103046471&origin=inward
In Vitro and In Vivo Antibacterial Activity of Gliotoxin Alone and in Combination with Antibiotics against Staphylococcus aureus
DOI: 10.3390/toxins13020085
LINK: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85100596463&origin=inward
Biological relevance of Granzymes A and K during E. coli sepsis
DOI: 10.7150/thno.59418
LINK: https://www.scopus.com/record/display.uri?eid=2-s2.0-85119990561&origin=resultslist&sort=plf-f&src=s&st1=10.7150%2fthno.59418&sid=fad97cd10a560299a1b5607079e5fee6&sot=b&sdt=b&sl=23&s=DOI%2810.7150%2fthno.59418%29&relpos=0&citeCnt=0&searchTerm=
Granzyme A inhibition reduces inflammation and increases survival during abdominal sepsis
ISSN:1838-7640
DOI: 10.7150/THNO.49288
URL: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85102460963&origin=inward
How could antibiotics, probiotics, and corticoids modify microbiota and its influence in cancer immune checkpoint inhibitors: A review
ISSN: 1098-5522
DOI: 10.1128/IAI.00665-20
URL: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85107008245&origin=inward
Integrated analysis of circulating immune cellular and soluble mediators reveals specific COVID19 signatures at hospital admission with utility for prediction of clinical outcomes
ISSN: 1838-7640
DOI: 10.7150/THNO.63463
URL: https://www.scopus.com/inward/record.uripartnerID=HzOxMe3b&scp=85120324638&origin=inward